Differences in Levels of Prostate Specific Antigen and Insulin-like Growth Factor 1 in GSTP1 Gene Polymorphism among Workers

Abstract

Cadmium (Cd) is classified as a carcinogen in humans (IA). In addition to causing lung cancer, the incidence of prostate cancer due to Cd exposure based on epidemiological research has also increased. This study aims to identify the GSTP1 genotypic frequency distribution and its correlation with PSA and IGF-1 levels in Cd exposed and unexposed workers. The research design used was cross sectional in 23 exposed groups (weld workers) and 40 unexposed groups (office employees) with a total of 63 people. The measurement of PSA and IGF-1 levels was carried out using the ELISA method and identification of the GSTP1 gene polymorphism using the PCR-RFLP method. Data analysis using Mann-Whitney and Spearman correlation test. The results showed that there was a relationship between PSA levels and IGF1 (p <0.01, r = 0.515) in all subjects. The results of identification of GSTP1 gene polymorphism were obtained as ile/ile genotypes as much as 30.2% and ile/val as much as 69.8%. There were no differences in PSA and IGF1 levels between ile/ile and ile/val genotypes (p> 0.05). There was a relationship between PSA and IGF1 (p <0.01, r = 0.569) in the group of Cd exposed workers and no differences in PSA and IGF1 levels between the ile/ile and ile/val genotypes (p> 0.05). There was a relationship between PSA and IGF1 (p <0.05, r = 0.342) in the unexposed group of workers and no differences in PSA and IGF1 levels between ile/ile and ile/val genotype (p>  0.05).